Mechanical properties of skin wounds after atropa belladonna application in rats


  • Tomáš Toporcer Department of Medical Biophysics, Faculty of Medicine, Pavol Jozef Šafárik University
  • Tomáš Grendel Department of Medical Biophysics, Faculty of Medicine, Pavol Jozef Šafárik University
  • Boris Vidinskỳ Department of Medical Biophysics, Faculty of Medicine, Pavol Jozef Šafárik University
  • Peter Gál Department of Medical Biophysics, Faculty of Medicine, Pavol Jozef Šafárik University
  • Ján Sabo Department of Medical Biophysics, Faculty of Medicine, Pavol Jozef Šafárik University
  • Radovan Hudák Department of Instrumental and Biomedical Engineering, Faculty of Mechanical Engineering, Technical University of Košice


wound healing, tensiometer, phytotherapy, inflammation, proliferation, maturation


Atropa belladonna is a topical agent used in the treatment of skin wounds in Slovak folk medicine. The aim of this study was to assess the effect of A. belladonna extract on wound tensile strength in Sprague-Dawley rats.

Twenty-four rats were randomly divided into 3 groups of 8 animals. Two symmetrical skin incisions were performed on the back of each animal and immediately sutured. In the 1st group A. belladonna extract was not applied and the group served as a control. The animals in the 2nd group were treated by daily application of A. Belladonna extract during the first two days after surgery. The animals in the 3rd group were treated during all five days after surgery. The wound tensile strength of each group was measured 120 hours after surgery. A separate group of 14 rats was used to measure the tensile strength of unwounded skin.

The mean tensile strength of both A. belladonna extract treated groups (2nd group: 244±48 g; 2.09±0.42% of unwounded skin; 3rd group: 254±67 g; 2.18±0.58% of unwounded skin) was significantly higher (p<0.05) than the untreated group (1st group: 194±31 g; 1.67±0.26 % of unwounded skin).

Results from our investigation suggest a positive effect of A. belladonna on aseptic surgical skin wound healing.


Download data is not yet available.


(1) Balzariny, A., Felisi, E., Martiny, A. and De Conno, F. 2000. Efficacy of homeopathic treatment of skin reaction during radiotherapy for breast cancer: a randomised, double-blind clinical trial. British Homeopathic Journal. 89 : 8-12.

(2) Biswas, T. K. and Mukherjee, B. 2003. Plant Medicines of Indian Origin for Wound Healing Activity: A Review Lower Extremity Wounds. 2 : 25-39.

(3) Burkitt, H. G., Quick, G. R. G. and Gatt, D. 1990. Essential Surgery. London, Churchill Livingstone,

(4) Connolly, A. J., Suh, D. Y., Hunt, T. K. and Coughlin, S. R. 1997. Mice lacking the thrombin receptor, Par1, have normal skin wound healing. Am. J. Path. 69: 1199-1208

(5) Gál, P., Kilík, R., Špaková, T., Pataky, Š., Sabo, J., Pomfy, M., Longauer, F. and Hudák, R. 2005. He-Ne laser irradiation accelerates inflammatory phase and epithelization of skin wound healing in rats. Biologia. (in press)

(6) Gillinzer, R. and Goebeler, M. 2001. Chemokines in cutaneous wound healing, J. Leukocyte Bio. 69 : 513-521

(7) Grieve, M.: A modern Herbal. [on line], 2004, available on: http://www. botanical/ mgmh/ n/ nighde05.html [cited 10-12-2004]

(8) Hudák, R., Gál, P., Toporcer, T., Kilík, R. and Sabo, J. 2004. Mechanical tensile strength tests of rat skin wounds during the first seven days of the healing process. J. Met. Mater. Miner. 14 : 11-19

(9) Kumar, V., Cotran, R. Z. and Robbins, S.L. 2003. Basic Pathology.7th ed.Philadelphia, Saunders.

(10) Maquart, F. X., Chastang, F., Simeon, A., Birembaut, P., Gillery, P. and Wegrowski, Y. 1999. Triterpenes from Centella asiatica stimulate extracellular matrix accumulation in rat exparimental wounds. Eur. J. Dermatology. 9 : 289-296.

(11) Moelleken, B. R. W. and Mathes, S. J. 1995. Plastic and Reconstructive Surgery. Yale University School of Medicine, Beverly Hills

(12) Rane, M. M. and Mengi, S. A. 2003. Comparative effect of oral administration and topical application of alcoholic extract of Terminalia arjuna bark on incision and excision wound in rats. Fitoterapia. 74 : 553-558.

(13) Rashed, A. N., Afifi, F. U. and Disi, A. M. 2003. Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in mus musculus JVI-1. J. Ethnopharmacology. 88 : 131-136.

(14) Rubin, E. and Faber, J. L. 1994. Pathology. New York, J.B. Lippincot.

(15) Samuelsen, A. B. 2000. The traditional uses, chemical constituents and biological activities of Plantago major L. A review. J. Ethnopharmacology. 71 : 1-21.

(16) Sperony, E., Govony, P., Guizzardi, S., Renzulli, C. and Guerra, M. C. 2002. Antiinflammatory and cicatrizing activity of Echinacea pallida Nutt. root extract. J. Ethnopharmacology. 79 : 265-272.

(17) Stadler, I., Raymond, J., Lanzafame, J., Evans, R., Narayan, V., Dailey, B., Buehner, N. and Naim, J. O. 2001. 830-nm Irradiation Increases the Wound Tensile Strength in a Diabetic Murine Model. Lasers in Surgery and Medicine. 28 : 220-226.

(18) Suguna, L., Singh, S., Sivakumar, P., Sampath, P. and Chandrakasan, G. 2002. Influence of Terminalia chebula on dermal wound healing in rats. Phytotherapy Research. 16 : 227-231.

(19) Whelan, H. T., Buchmann, E. V., Dhokalia, A., Kane, M. P., Whelan, N. T., WongRiley, M. T., Eells, J. T., Gould, L. J., Hammamieh, R., Das, R. and Jett, M. 2003. Effect of NASA Light-Emitting Diode Irradiation on Molecular Changes for Wound Healing in Diabetic Mice. J. Clinical Laser Med. Sur. 67: 67-74.




How to Cite

T. . Toporcer, T. Grendel, B. Vidinskỳ, P. Gál, J. Sabo, and R. Hudák, “Mechanical properties of skin wounds after atropa belladonna application in rats”, J Met Mater Miner, vol. 16, no. 1, Apr. 2017.



Original Research Articles