Improved L-929 cell growth from self assembled PDADMAC/gelatin thin films
Keywords:Layer-by-layer, L-929 fibroblast, chitosan, PDADMAC, gelatin
AbstractThis article demonstrates the advantages of using PDADMAC/gelatin over chitosan/gelatin multilayer thin films to improve L-929 mouse fibroblast growth. Although chitosan-based materials are often preferred in cell culture, we put in evidence the problematic low stability and decomposition of chitosan based film under physiological pH. Two kinds of coating were assembled using either chitosan or Poly(diallyldimethylammonium chloride) (PDADMAC) as polycationic polyelectrolyte and gelatin as the anionic counterpart. The non-toxic nature of each coating was confirmed by MTT assay for cell viability and cell proliferation. Using optical microscopy, it was observed that the L-929 fibroblast spread preferentially on the PDADMAC/gelatin surface when compared with the chitosan/gelatin surface. The lack of stability of the chitosan/gelatin films was put in evidence using atomic force microscopy (AFM) by monitoring the changes in thickness for each film when exposed to physiological buffer conditions. While PDADMAC/gelatin films were stable for several hours, chitosan/gelatin films presented a loss of thickness of nearly 40%. This loss of thickness could explain the observed detachment of the cell and poor spreading of the fibroblast cells when using the chitosan/gelatin coating.
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