Preparation and characterization of particles from chitosan with different molecular weights and their trimethyl chitosan derivatives for nasal immunization

Authors

  • Worawan Boonyo Faculty of Pharmaceutical Sciences, Naresuan University
  • Hans E. Junginger Faculty of Pharmaceutical Sciences, Naresuan University
  • Neti Waranuch Faculty of Pharmaceutical Sciences, Naresuan University
  • Assadang Polnok Faculty of Pharmaceutical Sciences, Naresuan University
  • Tasana Pitaksuteepong Faculty of Pharmaceutical Sciences, Naresuan University

Keywords:

Chitosan, degree of quaternization, molecular weight, nasal immunization, particle, trimethyl chitosan

Abstract

According to previous studies, molecular weights (MW) of chitosan presented an influence on the level of immune response to model antigen when administering with chitosan through nasal route. Moreover, trimethyl chitosan (TMC) with a degree of quaternization (DQ) of 40%, chitosan derivatives, was also shown to be the most potent nasal OVA delivery platforms compared to TMC with DQ of 20% and 60% in mice model. The aims of this study were to prepare particles from chitosan with difference MW and their TMC derivatives (DQ = 40%) and to characterize size, zeta potential, loading efficiency and release profile in order to be used as data for further studies as nasal adjuvant. The results showed that MW of chitosan in the studied range did not influence the particle characteristics. The particles prepared from TMC derivatives are of smaller size than those prepared from chitosan. However, they possessed higher zeta potential, loading efficiency and release profile than the particles prepared from chitosan.

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References

Davis, S.S. 2001. Nasal vaccines. Adv.Drug Delivery Rev. 51: 21-42.

Van der Lubben, I.M., Kersten, G., Fretz, M.M., Beuvery, C., Verhoef, J.C. and Junginger, H.E. 2003. Chitosan microparticles for mucosal vaccination against diphtheria: oral and nasal efficacy studies in mice. Vaccine. 21: 1400-1408.

Illum, L. 2003. Nasal drug delivery-possibilities, problems and solutions. J. Controlled Release. 87: 187–198.

Kotzé, A.F., Lueβen, H.L., de Leeuw, B.J., de Boer, B.G., Verhoef, J.C. and Junginger, H.E. 1997. N-trimethyl chitosan chloride as a potential absorption enhancer across mucosal surfaces: In vitro evalution in intestinal epithelial cells (Caco-2). Pharm. Res. 14(9): 1197-1202.

Illum, L., Jabbal-Gill, I., Hinchcliffe, M., Fisher, A.N. and Davis S.S. 2001. Chitosan as a novel nasal delivery system for vaccines. Adv. Drug Delivery Rev. 51 : 81-96.

Illum, L. 1998. Chitosan and its use as a pharmaceutical excipient. Pharm. Res. 15 : 1326-1331.

Kato, Y., Onishi, H. and Machida, Y. 2003. Application of chitin and chitosan derivatives in the pharmaceutical field. Current Pharm. Biotechnol. 4 : 303 – 309.

Boonyo, W., Junginger, H.E., Waranuch, N., Polnok, A. and Pitaksuteepong, T. 2007. Chitosan and trimethyl chitosan chloride (TMC) as adjuvants for inducing immune responses to ovalbumin in mice following nasal administration. J. Controlled Release. 121 : 168 – 175.

Polnok, A., Borchard, G., Verhoef, J.C., Sarisuta, N. and Junginger, H.E. 2004. Influence of methylation process on the degree of quaternization of N-trimethyl chitosan chloride. Eur. J. Pharm. Sci. 52 : 77-83.

Boontha, S., Junginger, H.E., Waranuch, N., Polnok, A. and Pitaksuteepong, T. 2008. Chitosan and trimethylchitosan particles for oral vaccine delivery: preparation and characterization. In : Proceeding of RGJ Seminar Series LIX: Nanotechnol. Drug Delivery. 55.

Kotzé, A.F., Thanou, M., Lueßen, H.L., de Boer, A.G., Verhoef, J.C. and Junginger, H.E. 1999. Effect of the degree of quaternization of Ntrimethyl chitosan chloride on the permeability of intestinal epithelial cells (Caco-2). Eur. J. Pharma. Biopharm. 47 : 269 – 274.

Sieval, A.B., Thanou, M., Kotzé, A.F., Verhoef, J.C., Brussee, J. and Junginger, H.E. 1998. Preparation and NMR characterization of highly substituted N-trimethyl chitosan chloride. Carbohydr. Polym. 36 : 157-165.

de Britto, D. and Campana-Filho, S.P. 2004. A kinetic study on the thermal degradation of N,N,N-trimethylchitosan. Polym. Degrad. Stab. 84 : 353-361.

Kean, T., Roth, S. and Thanou, M. 2005. Chitosans as non-viral gene delivery vectors: cytotoxicity and transfection efficiency. J.Controlled Release. 103 : 643 – 653.

Snyman, D., Hamman, J.H., Kotzé, J.S., Rollings, J.E. and Kotzé, A.F. 2002. The relationship between the absolute molecular weight and the degree of quaternisation of N-trimethyl chitosan chloride. Carbohydr. Polym. 50 : 145-150.

Amidi, M., Romeijn, S.G., Borchard, G., Junginger, H.E., Hennink, W.E. and Jiskoot, W. 2006. Preparation and characterization of proteinloaded N-trimethyl chitosan nanoparticles as nasal delivery system. J. Controlled Release. 111 : 107 – 116.

Sayin, B., Somavarapu, S., Li, X.W., Thanou, M., Sesardic, D., Alpar, H.O. and Şenel, S. 2008. Mono-N-carboxymethyl chitosan (MCC) and N-trimethyl chitosan (TMC) nanoparticles for non-invasive vaccine delivery. Int. J. Pharm. 363 (1-3) : 139- 148.

Boonsongrit, Y., Mitrevej, A. and Mueller, B.W. 2006. Chitosan drug binding by ionic interaction. Eur. J. Pharm. Sci. 62 : 267 - 274.

Chen, F., Zhang, Z.R. and Huang, Y. 2007. Evaluation and modification of N-trimethyl chitosan chloride nanoparticles as protein carriers. Int. J. Pharm. 336 : 166-173

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Published

2017-04-23

How to Cite

[1]
W. Boonyo, H. E. . Junginger, N. Waranuch, A. . Polnok, and T. Pitaksuteepong, “Preparation and characterization of particles from chitosan with different molecular weights and their trimethyl chitosan derivatives for nasal immunization”, J Met Mater Miner, vol. 18, no. 2, Apr. 2017.

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Original Research Articles

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