Effects of crosslinking agent and biological properties of silk fibroin/gelatin/chitosan ternary system electrospun nanofiber mats.
AbstractIn this study, electrospinning technique was used for ternary system to fabricate nanofiber mats from silk fibroin (SF):gelatin (G):chitosan (C) with various mass ratios i.e. 10:20:0, 10:20:0.5, 10:20:1, 10:20:1.5, 10:20:2, and 20:10:1. An increase in chitosan content of the mats was found to decrease average fiber diameter and with narrow size distribution. Tensile strength of SF:G:C nanofiber having greater SF content was lower than that of the fiber mat having lower SF content. The obtained fiber mats were then crosslinked by three different crosslinking agents including ethanol, glutaraldehyde and 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDC)/N-hydroxysuccinmide (NHS). The smooth fiber with high porosity of the nanofiber mat was observed after crosslinked using EDC/NHS agent. A less swollen fiber was noticed in the fiber mats having higher SF content. The results from in vitro study revealed the good cell adhesion and proliferation of gingival tissues. Such results indicated the potential use of SF:G:C nanofiber mats as membrane application.
How to Cite
LicenseAuthors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.