Immobilization of lipase onto <i>Cyperus Papyrus</i> L. for biodiesel production by transesterification and hydrolysis-esterification

Authors

  • Sireerat Charuchinda Faculty of Science, Chulalongkorn University, Biofuels by Biocatalysts Research Unit, Chulalongkorn University and Center for Petroleum, Petrochemicals, and Advanced Materials, Chulalongkorn University
  • Piyanan Suthianthong Faculty of Science, Chulalongkorn University
  • Warawut Chulalaksananukul Faculty of Science, Chulalongkorn University and iofuels by Biocatalysts Research Unit, Chulalongkorn University

Keywords:

Cyperus papyrus L., Candida rugosa lipase, Immobilization, Biodiesel, Transesterification, Hydrolysis-esterification

Abstract

Lipase of Candida rugosa was immobilized on biomass support, plant fiber from Cyperus papyrus L., for biodiesel synthesis. The two immobilization techniques investigated in this study were physical adsorption and covalent binding with 1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). Results showed that the prepared immobilized lipase by physical adsorption with adding heptane presented higher protein loading, lipase activity and degree of immobilization than that by physical adsorption in phosphate buffer solution and by covalent binding. This immobilized lipase was further applied as biocatalyst for biodiesel synthesis by transesterification and hydrolysis-esterification. The results showed that this immobilized lipase preferred to hydrolyze triglyceride in palm oil to be fatty acid in water as medium. Then, the obtained fatty acid could be a good substrate to react with alcohol for biodiesel synthesis by esterification route. So, the biodiesel synthesis yield by hydrolysis-esterification was higher than transesterification. Nevertheless, the results revealed that bioethanol was found to be a better substrate than methanol for biodiesel synthesis via enzymatic hydrolysis-esterification.

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Published

2022-08-13

How to Cite

[1]
S. Charuchinda, P. Suthianthong, and W. Chulalaksananukul, “Immobilization of lipase onto <i>Cyperus Papyrus</i> L. for biodiesel production by transesterification and hydrolysis-esterification”, J Met Mater Miner, vol. 21, no. 1, Aug. 2022.

Issue

Vol. 21 No. 1 (2011)

Section

Original Research Articles

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