Designing biphasic nanocellulose hydrogels to mimic the complex cartilage-bone interface

Sairash  SAIRASH; Sirinee JIRAJESSADA; Supansa YODMUANG

doi:10.55713/jmmm.v34i4.2066

Authors

Sairash SAIRASH Medical Science Program, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand
Sirinee JIRAJESSADA Biology Program, Faculty of Science, Buriram Rajabhat University, Muang, Buriram 31000, Thailand
Supansa YODMUANG Research Affairs, Faculty of Medicine, Chulalongkorn University, Bangkok 10330, Thailand; Center of Excellence in Biomaterial Engineering for Medical and Health, Chulalongkorn University, Pathumwan, Bangkok 10330, Thailand; Clinical Excellence Center for Advanced Therapy Medicinal Products, King Chulalongkorn Memorial Hospital, Pathumwan, Bangkok 10330, Thailand; Biomaterial Engineering for Medical and Health Research Unit, Chulalongkorn University, Bangkok 10330, Thailand

DOI:

https://doi.org/10.55713/jmmm.v34i4.2066

Keywords:

Bacterial cellulose, Hydroxyapatite, Biomineralization, Hydrogel, osteochondro

Abstract

Osteochondral lesions, which affect both the cartilage and the bone, present significant challenges in treatment due to the complex mechanical and biochemical properties of these tissues. A crucial consideration in developing tissue replacements for these lesions is the simultaneous regeneration of cartilage and calcified cartilage, which forms the transition zone to bone. Our current study aims to fabricate a bilayer polymeric hydrogel designed not only to support cartilage regeneration but also to serve as an interface between cartilage and bone. The bilayer hydrogel was created by combining oxidized bacterial nanocellulose, gelatin, and alginate in one layer, while the other layer consisted of the same three biopolymers and hydroxyapatite. The bacterial nanocellulose was effectively oxidized (20%) with sodium periodate and then mineralized with calcium and phosphorus (Ca/P ratio = 0.97), as confirmed by EDX analysis. Remarkably, both layers of the biphasic hydrogel demonstrated cytocompatibility with chondrocytes. Moreover, the addition of hydroxyapatite significantly improved the mechanical strength from 72 kPa (OBC/Gel/Alg) to 90 kPa (MOBC/Gel/Alg). This bilayer hydrogel holds promise for promoting bone-cartilage integration and has the potential to contribute to the healing of osteochondral defects, offering new possibilities in the field of orthopedic tissue engineering and regenerative medicine.

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