Chitin nanowhisker and chitosan nanoparticles in protein immobilization for biosensor applications
Keywords:
chitin, chitosan, nanoparticles, glucose oxidase, glucose biosensorAbstract
In this study, bovine serum albumin (BSA) was used as a model protein for the optimization of protein immobilization on chitin nanowhisker and chitosan nanoparticles under different conditions. Chitosan nanoparticles showed better results than chitinin this immobilization purpose. The immobilization at 15 minutes, pH 6 and 20-25°C was the optimal condition. Subsequently, glucose oxidase (GOD), enzyme used for glucose determination, was applied in order to investigate the optimum immobilization condition on chitosan nanoparticles. The optimal condition prove to...at pH 7, 30°C and 0.5 mg/ml of initial GOD concentration via immobilization at 15 minutes. Chitin nanowhisker and chitosan nanoparticles in this study had 300 and 39 nm in size, respectively. These results will be further used for the development of glucose electrodes.Downloads
References
Hirano, S., Seino, H., Akiyama, Y. and Nonaka, I. 1989. Biocompatibility of chitosan by oral and intravenous administration. Polym. Eng. Sci. 59 : 897–901.
Hirano, S., Seino, H., Akiyama, I. and Nonaka, I. 1990. Chitosan: a biocompatible material for oral and intravenous administration. In: Gebelein, C.G., Dunn, R.L. (eds.), Progress in Biomedical Polymers. New York : Plenum Press : 283–289.
Knapczyk, J., Krowczynski, L., Krzck, J., Brzeski, M., Nirnberg, E., Schenk, D. and Struszcyk, H. 1989. Requirements of chitosan for pharmaceutical and biomedical applications. In: Skak- Braek, G., Anthonsen, T., Sandford, P. (eds.), Chitin and Chitosan: Sources, Chemistry, Biochemistry, Physical Properties and Applications. London : Elsevier : 657–663.
Lin, J., Qu, W. and Zhang, S. 2006. Disposable biosensor based on enzyme immobilized on Au–chitosan-modified indium tin oxide electrode with flow injection amperometric analysis. Anal. Biochem. 360(2) : 288-293.
Luo, X.-L., Xu, J.-J., Du, Y. and Chen, H.-Y. 2004. A glucose biosensor based on chitosan–glucose oxidase–gold nanoparticles biocomposite formed by one-step electrodeposition. Anal. Biochem. 334(2): 284- 289.
Tang, Z.-X., Qian, J.-Q. and Shi, L.-E. 2007. Characterizations of immobilized neutral lipase on chitosan nano-porticles. Mater. Lett. 61 : 37 – 40.
Wu,Y., Yang, W., Wang, C., Hu, J., and Fu, S. 2005. Chitosan nanoparticles as a novel delivery system for ammonium glycyrrhizinate. Int. J. Pharm. 295 : 235–245.
Yang, M., Yang, Y., Liu, B., Shen, G. and Yu, R. 2004. Amperometric glucose biosensor based on chitosan with improved selectivity and stability. Sens. Actuators B: Chemical. 101 : 269 – 276.
Yin, B., Yuan, R., Chai, Y.Q., Chen, S.H., Cao, S.R., Xu, y. and Fu, P. 2008. Amperometric glucose biosensors based on layer-by-layer assembly of chitosan and glucose oxidase on the Prussian bluemodified gold electrode. Biotechnol. Lett. 30(2) : 317-322.
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