Chitin nanowhisker and chitosan nanoparticles in protein immobilization for biosensor applications
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chitin, chitosan, nanoparticles, glucose oxidase, glucose biosensorบทคัดย่อ
In this study, bovine serum albumin (BSA) was used as a model protein for the optimization of protein immobilization on chitin nanowhisker and chitosan nanoparticles under different conditions. Chitosan nanoparticles showed better results than chitinin this immobilization purpose. The immobilization at 15 minutes, pH 6 and 20-25°C was the optimal condition. Subsequently, glucose oxidase (GOD), enzyme used for glucose determination, was applied in order to investigate the optimum immobilization condition on chitosan nanoparticles. The optimal condition prove to...at pH 7, 30°C and 0.5 mg/ml of initial GOD concentration via immobilization at 15 minutes. Chitin nanowhisker and chitosan nanoparticles in this study had 300 and 39 nm in size, respectively. These results will be further used for the development of glucose electrodes.Downloads
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