Effect of Chemical Treatment on the Characteristics of Shrimp Chitosan

Authors

  • Pranee LERTSUTTHIWONG Bioprocess Technology, Metallurgy and Materials Science Research Institute, Chulalongkorn University
  • Ng Chuen HOW Bioprocess Technology, Metallurgy and Materials Science Research Institute, Chulalongkorn University
  • Suwalee CHANDRKRACHANG Bioprocess Technology, Metallurgy and Materials Science Research Institute, Chulalongkorn University
  • Willem F. STEVENS Bioprocess Technology, Metallurgy and Materials Science Research Institute, Chulalongkorn University

Keywords:

chitosan, deproteination, deacetylation, demineralization, chitin, viscosity

Abstract

The quality of chitosan produced from shrimp shell depends on the conditions of the chemical extraction process, including the concentration of chemicals used, the soaking time and the sequence of the treatments for deproteination, decalcification and deacetylation. The results show that 4% NaOH is a suitable concentration for removal of protein at room temperature (25°C). When the process started with deproteination, decalcification with 4% HCl for 2 h is sufficient to get chitin with a low ash content. For the production of chitosan with high viscosity, the decalcification should precede the deproteination. Chitosan with a high degree of deacetylation can be obtained by multiple deacetylation at 40°C.

Downloads

Download data is not yet available.

References

Bailey, J. L. 1967. Miscellaneous analytical methods. Techniques in protein chemistry. 2nd ed. Amsterdam, Elsevier Publishing.

Boyer, R. F. 1993. Modern experimental biochemistry. 2nd ed. California, The Benjamin Cummings Publishing : 51-57.

Chinadit, U., Wanichpongpan, P., Ng, C. H., Stevens, W. F. and Chandrkrachang, S. 1998. Chemical deacetylation of shrimp chitin in different conditions. In : Chen, Rong Huei and Chen, Hsing-Chen (eds.) Advances in Chitin Science. Vol. 3 : 165-168.

Department of Business and Economics. 1999. Trade statistics and economic indicators of Thailand. Ministry of Commerce, Thailand.

Hayes, E. R., Davies, D. H. and Munroe, V. G. 1977. Organic solvent systems for chitosan. Proceedings of 1st International Conference on Chitin and Chitosan. MIT Sea Grant Program. Massachusetts : 103. 020406080100012345678Time (days)DD (%) and solubility (%)00.40.81.21.62Ash content (%) DD (%)solubility (%)ash (%)

Jeuniaux, C. 1996. A brief survey of the early contribution of European scientists to chitin knowledge. In : Domard, et al.. (eds.) Advances in Chitin Science Vol. 1. Jacques.

Lertsutthiwong, P. 1997. Improved paper performance using chitosan. Master Thesis, Asian Institute of Technology, Pathumthani, Thailand.

National Food Institute 2001. Shrimp industry in Thailand at a glance. Bangkok, Thailand.

Ng, C. H., Chandrkrachang, S. and Stevens, W. F. 1996. Evaluation of the acid hydrolysis-HPLC method to determine the degree of acetylation for chitin and chitosan. Proceedings of 2nd Asia Pacific Symposium. Bangkok. S : 81-89.

Ng, C. H., Chandrkrachang, S. and Stevens, W. F. 2001. Effect of the deacetylation conditions on the distribution pattern of the N-acetamide groups in partially deacetylated chitin. In : Uragami, et al. (eds.). Chitin and Chitosan: Chitin and Chitosan in Life Science. Tokyo, Kodansha Scientific : 301-304.

No, H. K. and Meyers, S. P. 1997. Preparation of chitin and chitosan. In : Muzzarelli, R.A.A and Peter, M.G.(eds.) Chitin Handbook. Oxford, Pergamon Press: 475-489.

Pajak, A. W. and Bykowski, P. J. 1998. Production of chitosan with specified physicochemical properties by means of controlling the time and temperature of the reaction of deacetylation. Bulletin of the Sea Fisheries Institute. 1(143) : 75-81.

Poulicek, M., Foucart, M. F. V. and Jeuniaux, Ch. 1986. Chitinoproteic complexes and mineralization in mollusk skeletal structures. Chitin in Nature and Technology. New York, Plenum Press : 7-11.

Rao, M. S., Munoz, J. and Stevens, W. F. 2000. Critical factors in chitin production by fermentation of shrimp biowaste. Appl. Microbiol. Biotechnol. 54 : 808-813.

Roberts, G. A. F. 1992. Chitin Chemistry. London. The Macmillan Press.

Roberts, G. A. F. 1997. Chitosan production routes and their role in determining the structure and properties of the product. In : Domard, et al. (eds.)Advance in Chitin Sci. Vol. 2 Taiwan, National Taiwan Ocean University : 22-31.

Stevens, W. F. 2001. Production of chitin and chitosan: Refinement and sustainability of chemical and biological processing. In : Uragami, et al. (eds.) Chitin and Chitosan: Chitin and Chitosan in Life Science. Tokyo, Kodansha Scientific, et al. : 293-300.

Win, N. N., Pengju, G. and Stevens, W. F. 2000. Deacetylation of chitin by fungal enzymes. In : Uragani, et al. (eds.) Advance in Chitin Sci. Vol 4, Taiwan, National Taiwan Ocean University : 55-62.

Win, N. N. and Stevens., W. F. 2001. Shrimp chitin as substrate for fungal chitin deacetylase. Appl. Microbiol. Biotechnol. 57 : 334-341.

Downloads

Published

2022-08-21

How to Cite

[1]
P. LERTSUTTHIWONG, N. C. HOW, S. CHANDRKRACHANG, and W. F. STEVENS, “Effect of Chemical Treatment on the Characteristics of Shrimp Chitosan”, J Met Mater Miner, vol. 12, no. 1, pp. 11–18, Aug. 2022.

Issue

Vol. 12 No. 1 (2002)

Section

Original Research Articles

License

Copyright (c) 2002 The Journal of Metals, Materials and Minerals (JMMM)

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

Authors who publish in this journal agree to the following terms:

    1. Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgment of the work's authorship and initial publication in this journal.

 

    1. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal.