Enhancing stability and antioxidant efficacy of fisetin by encapsulating as β-cyclodextrin inclusion complex with porous polylactic acid film from breath figure

Charasphat PREUKSARATTANAWUT; Warinyupa  MANGMEE; Munchumas  PROUSOONTORN; Ekasit  NISARATTANAPORN; Krisana SIRALEARTMUKUL

doi:10.55713/jmmm.v31i1.1028

Authors

Charasphat PREUKSARATTANAWUT Innovative Metals Research Unit, Department of Metallurgical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand
Warinyupa MANGMEE Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Wangmai, Patumwan, Bangkok 10330, Thailand
Munchumas PROUSOONTORN Department of Biochemistry, Faculty of Science, Chulalongkorn University, Phayathai Road, Wangmai, Patumwan, Bangkok 10330, Thailand
Ekasit NISARATTANAPORN Innovative Metals Research Unit, Department of Metallurgical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, Thailand
Krisana SIRALEARTMUKUL Metallurgy and Materials Science Research Institute, Chulalongkorn University, Phayathai Road, Wangmai, Patumwan, Bangkok 10330, Thailand

DOI:

https://doi.org/10.55713/jmmm.v31i1.1028

Keywords:

Fisetin, Polylactic acid, DPPH assay, Porous film, Food and beverage products packaging

Abstract

This research aims to investigate the scope of fisetin (FIT)/β-cyclodextrin (β-CD) inclusion complexes with the ratios of 1:2 and 2:1 mole by means of freeze-drying, kneading and physical mixing methods (controlled process). Moreover, the complex compounds are analyzed by Fourier-Transform Infrared Spectroscopy (FTIR) and Differential Scanning Calorimetry (DSC). The results show that the most suitable preparation method of fisetin (FIT)/β-CD complex is the freeze-drying method with the fisetin (FIT) to β-CD ratio at 1:2. The emission of FIT/β-CD was investigated and it is found that the emission rate of freeze-dried FIT/β-CD in 1:2 ratio by freeze-drying method is lower than that by kneading and physical mixing methods. These results lead to an initiation of the innovative active packaging materials with synthetic polylactic acid (PLA) porous film by breath figure (BF) method in order to entrap FIT/β-CD inclusion complex. The honeycomb structure with and without the FIT/β-CD complex were analyzed by Scanning Electron Microscopy (SEM). Thereafter, the effectiveness of Antioxidant Activities (%AA) of the porous PLA films is measured by the anti-oxidation caused by 2,2-diphenyl-1-picrylhydrazyl (DPPH method). The result shows that PLA entrapping complex fisetin is more effective than PLA entrapping pure fisetin, by result of 53.0% and 48.6%, respectively.

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